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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Amino Acids. 2013 Jul 3;46(3):531–542. doi: 10.1007/s00726-013-1531-z

Figure 5.

Figure 5

Effect of SMO overexpression on H. pylori-stimulated iNOS expression, NO production, and bacterial killing. RAW 267.4 cells were transiently transfected with vector alone or vector containing SMO cDNA. Transfected cells were activated with HPL at an MOI of 100 for the times indicated. (A) mRNA expression assessed by RT-PCR at 6 h after stimulation with HPL. (B) Western blot analysis for iNOS (130 kDa) and β-actin (42 kDa). In A and B, data are representative of 3 separate experiments. (C) NO2− levels, measured by Griess assay. *p <0.05; **p < 0.01; ***p < 0.001 vs cells transfected with empty vector. Data are from 4 experiments. In D and E, live H. pylori placed above Transwell filter supports was incubated for 24 h at an MOI of 100 with macrophages transfected with vector alone or vector containing SMO cDNA. CFU were determined after 24 h of coculture. (D) H. pylori survival. (E) NO2− levels in culture supernatants. **p < 0.01, ***p < 0.001 vs no macrophages; and §§p < 0.01 vs empty vector-transfected macrophages. Data are from 2 separate experiments performed in duplicate.