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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Amino Acids. 2013 Jul 3;46(3):531–542. doi: 10.1007/s00726-013-1531-z

Figure 6.

Figure 6

Levels of ODC, SMO mRNA and spermine in HPL-activated human monocytic cell lines and effect of SMO overexpression on H. pylori-stimulated NO. (A and B) THP-1 cells were activated with HPL activated with HPL at an MOI of 100 for the times indicated. (A) mRNA expression assessed by real-time PCR at 6 h after stimulation with HPL. (B) Levels of spermine at 24 h after stimulation with HPL. (C and D) U937 and THP-1 cells were transiently transfected with vector alone or with SMO plasmid vector and activated with HPL at an MOI of 100 for 72 h. NO2− levels, measured by Griess assay for (C) U937 cells and (D) THP-1 cells. *p < 0.05, **p < 0.01; vs unstimulated control cells transfected with empty vector; §p < 0.05, §§p < 0.01 vs HPL-stimulated cells transfected with empty vector. Data are from 3 experiments.