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. Author manuscript; available in PMC: 2014 Nov 1.

Published in final edited form as: JPEN J Parenter Enteral Nutr. 2013 May 2;37(6):10.1177/0148607113486931. doi: 10.1177/0148607113486931

Histological H&E stain of gonadal adipose tissue from mice fed high- or low-fat diet. Gonadal depots were excised, fixed in 4% formalin/PBS, and paraffin embedded. 5-micron sections were processed and are representative of n=3-4 per diet. Adipocyte areas were calculated using ImageJ software (described in Methods) quantifying 12 representative adipose cells in each section per diet group. No statistically significant difference was observed between mean adipocyte areas between each diet group (P>0.05).

Histological H&E stain of gonadal adipose tissue from mice fed high- or low-fat diet. Gonadal depots were excised, fixed in 4% formalin/PBS, and paraffin embedded. 5-micron sections were processed and are representative of n=3-4 per diet. Adipocyte areas were calculated using ImageJ software (described in Methods) quantifying 12 representative adipose cells in each section per diet group. No statistically significant difference was observed between mean adipocyte areas between each diet group (P>0.05).