Figure 4.2.

An engineered cartilage construct (10 mm diameter × 2.3 mm thick) is cultured in a 30 mm diameter dish containing 2.5 mL of media. The initial concentration of glucose is 25 mM and media is refreshed thrice a week, on a 2-2-3 day schedule. The culture media is assumed to be well stirred. Glucose diffuses from the bath into the construct and is taken up by chondrocytes at a rate guided by Michaelis-Menten kinetics Sengers et al. (2005).