Fig. 7.

PA decreases AMPK/TSC2 phosphorylation. C2C12 myocytes were incubated overnight in the presence or absence of EtOH as described in Fig 5. Equal amounts of protein from cell lysates were analyzed via Western blotting using antibodies against phosphorylated (p) forms of AMPKα (A) and TSC2 (B). The data shown are mean ± SE of 3 independent experiments consisting of 3–4 replicate samples per experiment. Groups with different letters are significantly different from one another (P< 0.05). Groups with the same letters are not significantly different.