Fig. 8.

PA enhances the interaction of mTOR with Rheb and Rag GTPases. C2C12 myocytes were incubated in the presence or absence of EtOH as described in Fig. 5. mTOR was immunoprecipitated from equal amounts of cell extract and then immunoblotted with antibodies as indicated (A). Results were quantified as bar graphs (B–D) and normalized with immunoprecipitated (IP) mTOR that was assessed by immunoblotting. The data shown are mean ± SE of 3 independent experiments consisting of 3–4 replicate samples per experiment. Groups with different letters are significantly different from one another (* P< 0.05). Groups with the same letters are not significantly different.