C57Bl/6 and CaMKK−/− mice were subjected to cecal ligation and puncture, followed by normal saline (3 mL/100 gm) and imipenem/cilastatin (0.5 mg/kg) administration. After 12 hours, mice were administered CaCl2 (2.5 mg/kg) or equivolume normal saline by intraperitoneal injection. After 24 hours, mice were euthanized, and blood was isolated by cardiac puncture, and the serum analyzed for alanine aminotransferase (ALT) concentrations and blood urea nitrogen (BUN). (n=12 animals per group)