C57Bl/6 and CaMKK−/− mice were subjected to cecal ligation and puncture, followed by normal saline (3 mL/100 gm) and imipenem/cilastatin (0.5mg/kg) administration. After 12 hours, mice were administered CaCl2 (2.5 mg/kg) or equivolume normal saline by intraperitoneal injection. After 12 hours, each organ was harvested and vascular permeability assessed by Evans Blue dye as detailed in the Methods (n=6 animals per group). Top panel represents data from liver, middle panel represents data from kidney, and bottom panel represents data from lung.