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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Mol Biol. 2013 May 4;425(22):10.1016/j.jmb.2013.04.027. doi: 10.1016/j.jmb.2013.04.027

Fig. 6.

Fig. 6

The NCA spectra of free U-13C,15N CAP-Gly (A) and U-13C,15N CAP-Gly in complex with EB1 (B) acquired at 19.9 T. The labels of the cross peaks corresponding to T43, V44, and A45 are shown in purple. In (C) and (D), the overlaid 2D NCACX spectra of free CAP-Gly and CAP-Gly in complex with EB1 illustrate: (C) the two conformers of T43a and T43b in free CAP-Gly (black) and a single peak of T43 (purple) of CAP-Gly in complex; (D) the two conformers of A45a and A45b in free CAP-Gly (black) and a single peak of A45 (purple) of CAP-Gly in complex. In (E), (F) and (G), the overlaid inserts of NCOCX spectra of free CAP-Gly and CAPGly in complex are presented, illustrating the sequential cross peaks corresponding to two conformers in free CAP-Gly: V44a-T43a and V44b-T43b, A45a-V44a and A45b-V44b (all shown in black), and to a single conformer of CAP-Gly in complex with EB1 V44-T43 and A45-V44 (shown in purple).