LFH calls emitted by female mice do not differ with context. A, Spectrograms and amplitude envelopes of two series of LFH calls emitted during mating and agitation. Open arrows indicate LFH calls. Both long and short LFH calls were emitted in both contexts. During the mating context, ultrasonic mating vocalizations likely produced by the male mouse are also observed (solid arrows). B, Mean and 95% confidence intervals for duration of LFH calls in the two contexts. There were no significant contextual differences. C, Mean and 95% confidence intervals of the frequency contours of LFH calls emitted during mating and agitation. D, Spectrogram and amplitude envelope of the exemplar used for the study of approach behavior and for amygdalar electrophysiology.