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Relative cell viability of transduced human adipose tissue derived stem cells compared to untransduced human adipose tissue derived stem cells determined by MTT assay. In the horizontal axis, the first value 0.0 is related to untransduced human adipose tissue derived stem cells (hADSCs) without any virus transduction. The optimum volume of non-integrated lentiviruses harboring PDX1 was obtained as 0.8 μL/15000 hADSCs that has been used for cell transductions.
