Figure 6.

Morphological differentiation of human adipose tissue derived stem cells^PDX1+ three weeks after second transduction cultured in differentiation medium containing high glucose Dulbecco’s modified Eagle’s medium supplement by B27, nicotinamide and βFGF. A: Untransduced human adipose tissue derived stem cells (hADSCs) were a typically adherent spindle shape; B: Human ADSCs^PDX1+ became oval-type cells after second transduction; C: Human ADSCs^PDX1+ formed clusters in the medium containing high glucose Dulbecco’s modified Eagle’s medium (DMEM), B27 and nicotinamide 7 d after transduction; D: Human ADSCs^PDX1+ continued to differentiate and formed larger clusters in 3 wk in the medium containing high glucose DMEM, B27, nicotinamide and βFGF.