Table 1.
Sequences of primers used in real-time reverse transcription-polymerase chain reaction and polymerase chain reaction experiments
| Gene | Gene ID | Size (bp) | Strand | Sequence (5'–3') | Annealing temperature |
| Somatostatin | NM_001048.3 | 114 | F | GCACCCCGAGAACGCAAAGC | 56 |
| R | TGTGGGGGCGAGGGATCAGAG | ||||
| Ngn3 | NM_020999.3 | 241 | F | GTAGAAAGGATGACGCCTCAACC | 54 |
| R | TCAGTGCCAACTCGCTCTTAGG | ||||
| Glucagon | NM_002054.4 | 187 | F | CAGTGCGCCTTGGTGCAGAAGT | 58 |
| R | GGAACGTTGCCAGCTGCCTTG | ||||
| Glut2 | NM_000340 | 300 | F | AGCTTTGCAGTTGGTGGAAT | 52 |
| R | AATAAGAATGCCCGTGACGA | ||||
| PDX1 | NM_000209.3 | 201 | F | GTCCGGTGCCAGAGTTCAGT | 63 |
| R | CCCAGTCTCGGTTCCATTCG | ||||
| β-actin | NM_001101.3 | 161 | F | GAGACCTTCAACACCCCAGCC | 56 |
| R | AGACGCAGGATGGCATGGG |
Ngn3: neurogenin3; Glut2: glucose transporter2.