Fig. 1.

Differential induction of tyrosine phosphorylation of DCC and neogenin by netrin-1. a Tyrosine phosphorylation of DCC and neogenin in netrin-1-stimulated rat cortical neurons (E17, DIV3). Neuronal lysates (500 μg of protein) were incubated with anti-DCC or neogenin and immunoprecipitated DCC or neogenin was subjected to Western blot using the antibody RC20 and anti-DCC or neogenin (as control). b Netrin-1 induction of tyrosine phosphorylation of DCC, but not neogenin, in HEK 293 cells expressing FAK. HEK 293 cells were co-transfected Myc-FAK or PYK2 with Flag-DCC, or neogenin, and stimulated with netrin-1 for 30 min. Cell lysates were incubated with an anti-Flag antibody for immunoprecipitation and immunoprecipitated proteins were probed with indicated antibodies.