Figure 2. Lack of CD200R signal results in enhanced macrophage VEGF expression in response to RPE-derived PGE₂.

(A) PGE₂ secretion from murine B6RPE-07 cells cultured for 6–24 hr was determined by ELISA. (B) Vegf mRNA expression from BMMΦ cultured for 6 hr in RPE conditioned media collected after 24 hr. 18srRNA was used as internal control. (C) Relative mean fluorescent intensity (MFI) of VEGF expression in WT or CD200R1^−/−macrophages. (D) Representative confocal images of BMMΦ cultured in RPE supernatants or control media for 24 h. Cells were fixed and permeabilized before immuno-staining with anti-VEGF antibody. Scale bar: 20 μm. Data are presented as mean ± SEM, n≧3. *P < 0.05, **P < 0.005 between groups.