Table 1.

Singlet Oxygen Bubbles Causing the Inactivation of Escherichia coli and Aspergillus fumigatus^a

	E. coli inactivation				Asp. fumigatus inactivation
pre-equilibrium condition	E. coli (μg/mL)	volume (mL)	% killed after 120 min	number of cells killed per bubble	Asp. fumigatus (μg/mL)	volume (mL)	% killed after 120 min	number of cells killed per bubble
nitrogen-presaturationb	30	3	90±5	114	30	3	88±5	111
	15	10	69±4	87
					10	3	70±2	88
	3	30	60±2	76
air-presaturation	30	3	63±5	80	30	3	62±5	78
	15	10	57±4	72
					10	3	50±4	63
	3	30	50±4	63
oxygen-presaturationc	30	3	8±3	10	30	3	3±1	4
	15	10	5±1	6
					10	3	2±0.5	2
	3	30	3±1	4

^aLaser light (669 nm) and O₂ gas (60 mL/min) were directed into the device. 90 μL bubbles emerged from the device into aqueous solution. Bubble sizes were measured from photographic images with pixel size correlations, as well as a ruler as a reference point.

^bSamples received the ¹O₂ bubbles from the device after first being deaerated by purging with N₂ (oxygen concentration = 0.012 mM; 0.4 ppm).

^cSamples received the ¹O₂ bubbles from the device after first being aerated by purging with O₂ (oxygen concentration = 0.83 mM; 26.6 ppm).