Figure 4. RecBCD and AdnAB are repair pathway specific nucleases.

A. Repair outcomes for ΔrecBCD, ΔadnAB, and ΔadnABΔrecBCD strains carrying the no homology I-SceI repair substrate (see Figure 1A). The data for WT strain are the same as that presented in Figure 1D.

B. Repair outcomes for the indicated strains and representative hygromycin/X-gal plates using the recombination substrate pictured in Figure 3A. The table shows the mean % survival and % blue of three independent experiments (with standard error of the mean, SEM, indicated in parentheses). The number of repair outcomes among white and blue colonies for each strain used to calculate the percentages graphed in the pie graphs are shown and are derived from a single representative experiment. The data for WT strain are the same as that presented in Figure 3D.