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. Author manuscript; available in PMC: 2013 Oct 30.
Published in final edited form as: Curr Protoc Cell Biol. 2009 Dec;0 4:10.1002/0471143030.cb0424s45. doi: 10.1002/0471143030.cb0424s45

Figure 4. Autocorrelation on plasma membrane associated GPI-GFP.

Figure 4

Autocorrelation function is collected from in a confocal volume chosen on the plasma membrane, where GFP is anchored via a GPI domain (A). Note how the baseline for the autocorrelation function is not flat (between 100ms and 1s) because of photobleaching of the plasma membrane-anchored GFP (B). Photobleaching is apparent as an exponential decay in the countrate (Inset C).