Figure 3.

Immunocytochemistry and Western blot analysis of the identification of CAFs/NF. CAFs positive forα-SMA positive (A, brown), and all the NFs were negative for α-SMA (B). Both the CAFs and the NFs expressed, vimentin (C, D: brown), but lacked CK (pan) expression (E, F). Few CK (pan) positive epithelial cells were mixed with NF in Figure F. Figure G shows the result of Western blot analysis for validation of α-SMA expression. Western blot analysis confirmed the α-SMA expression in CAFs and but not in NFs. A, B, E, F 50-fold; C, D 200-fold.