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Averaged b0 images (from anterior to posterior) for a single subject acquired using the standard high-resolution protocol, with red arrows indicating the positions of the optic nerves, red indicating the positioning of the 4 pixels included in each of the ROIs, and a blue arrow indicating the optic chiasm. The anterior part of the optic nerve is easiest to observe, whereas its middle portion can become obscured due to the wrap-around artifact in posterior image slices.
