Figure 3.

Cell surface phenotypes of CD59^–CD34⁺ and CD59⁺CD34⁺ PNH cell populations prior to culturing. Each cell population was examined for CD38 (a and b), CD19 (c and d), CD64 (e and f), or CD71 (g and h) in three-color analyses as described in Methods. For each marker, a total of 20,000 gated events was collected based on forward and side scatter and cells were analyzed gating on CD34 positivity and CD59 positivity or negativity. In each instance about 75–100 events were recovered.