Figure 3.

Activation of caspase-3 in OLGs after hypoxia. All the samples of hypoxia-treated OLGs were prepared 6 hours after hypoxia. (a) Western blot analysis showing that the cleavage of procaspase-3 occurred in wild-type, but not in Cre/p35, OLGs after hypoxia. (b and c) Under normal conditions, WT-OLGs did not exhibit immunoreactivity for the active form of caspase-3. (d and e) WT-OLGs showing immunoreactivity for the active form of caspase-3 and a condensed nuclear morphology. Note that a neighboring OLG that did not display immunoreactivity for the active form of caspase-3 had a normal nuclear morphology even after hypoxia (arrows). Original magnification, ×200. (f) Hypoxia-induced caspase-3–like protease (DEVDase) activation in wild-type and Cre/p35 cells. The measurements were carried out in triplicate for two different samples. The changes in DEVDase activity after hypoxia were expressed as a percentage. Statistical analysis was performed with a nonpaired t test. ^AP < 0.01. (g) Hypoxia-induced caspase-1–like protease (YVADase) activation in wild-type and Cre/p35 OLGs. The changes in YVADase activity after hypoxia were expressed as a percentage (means ± SEM). The measurements were carried out in triplicate for two different samples.