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. 2000 Sep 1;106(5):681–688. doi: 10.1172/JCI10318

Figure 6.

Figure 6

(a) Decreased extracellular [Cl–] increases pp38 expression in cultured cTAL cells. Cultured cTAL cells were incubated in the indicated solution for 6 hours. (lane 1) control; (lane 2) low salt; (lane 3) Na isethionate; (lane 4) choline chloride; (lane 5) NaCl. AP < 0.01 compared with control; n = 5. Inset: representative experiment. (b) Decreased extracellular [Cl-] increases p38 kinase activity in cTAL cells. cTAL cells were incubated in different media for 6 hours, and p38 kinase activity was measured as described in Methods, using ATF2 as substrate. (lane 1) control; (lane 2) low salt; (lane 3) NaCl; (lane 4) Na isethionate; and (lane 5) choline chloride. (c) Time course of pp38 expression in cTAL incubated in low-salt medium. Cells were incubated in low-salt medium 0–16 hours (n = 3). AP < 0.01 compared with base line. (d) Time course of pJNK and pERK expression in cTAL incubated in low-salt medium.