Figure 5.

Western blot analysis of R1α and R2β in normal lymphoblasts, Carney complex lymphoblasts, and Carney complex cardiac myxoma. (a) Positive controls for antibodies to R1α and R2β were electrophoresed and Western blotted as described in Methods. (b, c) Protein lysates of lymphoblasts from (b) a normal individual and (c) an individual affected by Carney complex (family YA, IV-1) were electrophoresed and analyzed. Densitometry revealed similar levels of R2β protein in the two samples but a 60% decrease in R1α levels. (d) R1α and R2β were analyzed in lysate from a left atrial myxoma resected from individual IV-1 in family YA. Densitometry was used to determine R1α and R2β expression in all samples, and the ratio of R1α:R2β was calculated. A reversal of the R1α:R2β ratio is observed in the tumor sample compared with affected and unaffected lymphocytes.