Table 2. Calculated Imino Proton Exchange Rates in the Absence of Added Catalyst and Equilibrium Constants for Base Pair Opening, for Base Pairs G3:C10, A4:T9, G5:C8, and C6:G7 in the OL-1a and OL-1b Duplexesa.
|
kex (s–1)b |
αKop ×
106 |
|||
|---|---|---|---|---|
| base | OL-1a | OL-1b | OL-1a | OL-1b |
| G3 | 0.8 ± 0.1 | 0.5 ± 0.1 | 4.1 ± 0.2 | 3.0 ± 0.04 |
| T9 | 1.1 ± 0.05 | 0.5 ± 0.03 | 13.8 ± 0.1 | 8.8 ± 0.2 |
| G5 | 0.5 ± 0.06 | 0.4 ± 0.04 | 0.20 ± 0.002 | 0.20 ± 0.004 |
| G7 | 0.7 ± 0.08 | 0.5 ± 0.03 | 0.20 ± 0.002 | 0.40 ± 0.007 |
a
Measured at 15 °C.
b
The observed exchange rate without addition of ammonia catalyst.