. Author manuscript; available in PMC: 2013 Oct 30.

Published in final edited form as: J Mol Biol. 2005 Dec 12;356(3):10.1016/j.jmb.2005.11.077. doi: 10.1016/j.jmb.2005.11.077

Table II.

Results from FRET data fits with two variable parameters: free energy of dimerization, ΔG (kcal/mole) and Förster radius, R₀ (Å).

	Donor/Acceptor Pair	ΔG (kcal/mole)	R₀ (Å)
^RRTM	Fl/Rhod	−2.7	55.4
	BODIPY-Fl/Rhod	−2.8	55.8
	combined	−2.8	54.7

^RRTM(E)	Fl/Rhod	−4.4	52.1
	BODIPY-Fl/Rhod	−4.0	53.6
	combined	−4.1	53.4

The calculated ΔG provided the best fit to the experimental data shown in Figure 6B (see text for details). The results do not depend on the initial guesses for ΔG and R₀, indicative of unique robust fits.