Table 1. ELISA IC50 values, obtained by competition against sCD4 on gp120SF162 and gp120CN54, of the miniCD4s belonging to the M48 series.
NO | R | gp120SF162 IC50 (nM ± SEM) | gp120CN54 IC50 (nM ± SEM) | A: gp120SF162 |
B: gp120SF162 |
|
B/A | ||
---|---|---|---|---|---|---|---|---|---|
1 | M48c | 0.52 ± 0.04 | 1.9 ± 0.1 | 1 | 1 | 4 | 1 | ||
2 | M48U1c | 0.35 ± 0.07 | 0.37 ± 0.04 | 0.7 | 0.13 | 1 | 0.2 | ||
3 | M48U2c | 0.50 ± 0.09 | 1.7 ± 0.3 | 0.9 | 0.59 | 3 | 0.7 | ||
4 | M48U3c | 0.32 ± 0.06 | 11.7 ± 0.1 | 0.6 | 4.0 | 37 | 6.7 | ||
5 | M48U4* | 1.13 ± 0.18 | 6.6 ± 1 | 2.1 | 2.3 | 6 | 1.1 | ||
6 | M48U5* | 5.17 ± 1.26 | 50.2 ± 5.7 | 9.8 | 17 | 10 | 1.7 | ||
7 | M48U6* | 0.86 ± 0.07 | 10.1 ± 1.6 | 1.6 | 3.5 | 12 | 2.2 | ||
8 | M48U7* | 0.30 ± 0.06 | 2.4 ± 0.4 | 0.6 | 0.83 | 8 | 1.4 | ||
9 | M48U8* | 0.28 ± 0.05 | 3.4 ± 1.1 | 0.5 | 1.2 | 12 | 2.4 | ||
10 | M48U9* | 5.28 ± 0.46 | 38.2 ± 4.6 | 10 | 13 | 7 | 1.3 | ||
11 | M48U10* | 284 ± 21 | 13076 ± 415a | 536 | 4509 | 46 | 8.4 | ||
12 | M48U11* | 2026 ± 277 | 12061 ± 27a | 3823 | 4159 | 6 | 1.1 | ||
13 | M48U12* | 0.26 ± 0.05 | 0.31 ± 0.05 | 0.5 | 0.11 | 1 | 0.2 | ||
14 | M48U13b,c | 1.40 ± 0.46 | 15.5 ± 1.1 | 2.6 | 5.3 | 11 | 2 | ||
15 | M48U14* | 8.1 ± 1.6 | 1230 ± 180 | 15 | 424 | 152 | 28 |
Extrapolated values (maximum concentration tested was 10 mM).
Mentioned in reference (19).
New compounds. IC50 values are presented as the mean ± SEM values from three independent experiments.