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. 2013 Oct 28;203(2):197–204. doi: 10.1083/jcb.201307040

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© 2013 Dulle et al.

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Figure 1. — hsp104-R830S propagates cryptic [PSI+]. (A) [PSI+], [psi−], and hsp104-R830S cells were spotted on media containing CuSO₄ to induce toxic overexpression of Sup35, media lacking adenine (SD-Ade) to assess nonsense suppression of the premature stop codon in ade1-14, and rich media (YPD). (B) HSP104 [PSI+] and hsp104-R830S cells containing pSup35NM-GFP were imaged by fluorescence microscopy. Bar, 10 µm. (C) Western blot of an SDS-containing agarose gel (SDD-AGE) shows Sup35 aggregate status in lysates of indicated strains. This blot is one representative of three individual experiments. (D) An example of two tetrads where mating hsp104-R830S to [psi−] cells, both containing the ade1-14 mutation, resulted in tetrads with two red (efficient translation termination, hsp104-R830S) and two light pink (increased nonsense suppression, [PSI+] HSP104) haploids.