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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Sep 1;20(10):1156–1163. doi: 10.1038/nsmb.2656

Figure 3. AKAP95 is associated with DPY30-MLL complexes.

Figure 3

(A) Coomassie blue-stained 4–20% SDS-PAGE gel of proteins eluted from M2 agarose beads. Major proteins identified specifically from the FH-DPY30 cell line by MALDI mass spectrometry are indicated. Lines on the right of the gel indicate positions of gel excision for mass spectrometry.

(B) Immunoblot analysis of endogenous DPY30-MLL complexes following IP of HeLa cell nuclear extract by anti-DPY30 or normal rabbit IgG antibodies. Refer to Supplementary Fig. 8B.

(C) Immunoblot analysis of DPY30-MLL complexes following IP by M2 beads of lysates of 293T cells transiently transfected with or without FLAG-HA-WDR5 plasmid. Refer to Supplementary Fig. 8C.

(D) Immunoblot analysis of the purified FH-DPY30 complexes following fractionation by gel filtration on Superose 6. Elution volumes are indicated at the top.