Figure S. 2.

A) TLC bioautography on dichloromethane (DCM) extract of the algae (100 μg/spot; 1) Sargassum boveanum 2) Cystoseira myrica and 3) Hypnea flagelliformis) on a silica gel plate, and the plate immersed in Staphylococcus aureus suspension and after incubation at 37 ºC sprayed with iodonitrotetrazolium chloride solution (INT). The pale zones indicated the presence of antibacterial compounds. B) Silica gel TLC using 5% acetone in chloroform as the mobile phase visualised by vanillin-sulphuric acid solution which was then used as a guide to separate the antibacterial constituents on the preparative-TLC in three bands for the algal extracts. Hyp stands for Hypnea flagelliformis