Table 1.
Comparison results of fed-batch culture E.coli BL21 (DE3) (pET3a-hifnγ) under optimum condition (2.25 × 10-3 g g-1 l-1 IPTG per DCW, DCW = 65 g l-1 as induction time, post-induction duration 4 h) and non-optimized (11 mg g-1 l-1, DCW = 65 g l-1 as induction time, post-induction duration 3 h).
| Parameters | Optimum condition |
Non-optimum condition
(previous work) (22) |
|---|---|---|
| Cultivation time (h) | 17 | 16.5 |
| Total glucose consumption (g) | 295 | 312 |
| Total ammonium solution 25%(w/v) consumed (g) | 123 | 142 |
| Final cell density (g l-1 DCW) | 127 | 112 |
| Productivity of biomass g l-1 h-1 (DCW) | 7.65 | 7 |
| Final concentration of rhIFN-γ (g l-1) | 51 | 42 |
| Yield of rhIFN-γ/ biomass, Yp/x g g-1 (rhIFN-γ per DCW) | 0.4 | 0.37 |
| Yield of rhIFN-γ/ substrate, Yp/s g g-1 (rhIFN-γ per glucose) | 0.17 | 0.16 |
| Yield of biomass/ substrate, Yx/s g g-1 (DCW per glucose) | 0.424 | 0.43 |
| Productivity of rhIFN-γ g l-1.h-1 | 3 | 2.57 |