Table 12.
Buffer for P1-T Adaptor Ligation
| Components | Final concentration | Per reaction |
|---|---|---|
| TE | - | 5 ul |
| 10x T4 DNA ligase buffer | 1x | 3 ul |
| P1 Adaptor (15 uM) | 0.4 uM | 1 ul |
| T4 DNA ligase (500 U/ul) | 500 U | 1 ul |
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