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Buffer for P2-T Adaptor Ligation on Beads
| Components | Final concentration | Per reaction |
|---|---|---|
| Chromatin on beads | - | 20 ul |
| 10 mM Tris-HCl (pH 7.5) | 10 mM | 28 ul |
| 10x T4 DNA ligase buffer | 1x | 6 ul |
| P2-T Adaptor or P2 Adaptor (15 uM) | 1.25 pM | 5 ul |
| T4 DNA ligase (500 U/ul) | 500 U | 1 ul |
Total volume = 60 ul
