Table 8.
Buffer for RecJf Exonuclease Digestion on Beads
| Components | Final concentration | Per reaction |
|---|---|---|
| Chromatin on beads | - | 20 ul |
| 10 mM Tris-HCl (pH 8.0) | 10 mM | 33 ul |
| 10x NEBuffer 2 | 1x | 6 ul |
| RecJf Exonuclease (30 U/ul) | 30 U | 1 ul |
Total volume = 60 ul
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