Table 1.
Summary of all eligible studies examining the association between biomarker expression and survival in gemctiabine-treated pancreatico-biliary cancer patients.
| Marker | Reference | Year | n | Assay/ Specimen |
Assay Cutpoints (n) | Cancer Type |
|---|---|---|---|---|---|---|
| hENT1 | Kondo (28) |
2011 | 86 | IHC FFPE |
Low (23) or high (63) on a 0-3 score system; high defined as score >=2 in > 50% cells. |
Pancreas |
| Morinaga (31) |
2011 | 27 | IHC FFPE |
Low (11) and high (16) on a 6-point score system; cutoff point at mid-point of the score system. |
Pancreas | |
| Murata (32) |
2011 | 55 | IHC FFPE |
Negative (16) or positive (39) on a 0-3 score system; negative defined as score =0 or =1 in >50% cells. |
Pancreas | |
| Fujita (24) |
2010 | 40 | qPCR FFPE |
Low (26) or high (14); cutoff determined by recursive descent partition analysis. |
Pancreas | |
| Farrell (23) |
2009 | 91 | IHC FFPE |
Negative (18) or positive (73); negative defined as no staining in > 50% cells. |
Pancreas | |
| Marechal (29) |
2009 | 45 | IHC FFPE |
Low (26) or high (19) on a 0-300 staining score system; cutoff point at the median. |
Pancreas | |
| Giovannetti (25) |
2006 | 81 | qPCR Frozen |
Low (27), medium (28), or high (26) gene expression level; tertile cutoff. |
Pancreas | |
| Spratlin (37) |
2004 | 21 | IHC FFPE |
Negative (12) or positive (9) on a 0-2 score system; negative defined as 0. |
Pancreas | |
| Santini (35) |
2011 | 31 | IHC FFPE |
Negative (10) or positive staining (21) on a 0-2 score system; positive defined as > 50% cell stained. |
Bile duct | |
| Borbath (22) |
2011 | 43 | IHC FFPE |
Low (9) or high (17); low defined as no staining in > 50% cells. |
Bile duct | |
|
| ||||||
| dCK | Fujita (24) |
2010 | 40 | qPCR FFPE |
Low (27) or high (13); cutoff determined by recursive descent partition analysis. |
Pancreas |
| Marechal (30) |
2010 | 45 | IHC FFPE |
Low (26) or high (19) on a 0-200 staining score system; cutoff at median. |
Pancreas | |
| Giovannetti (25) |
2006 | 81 | qPCR Frozen |
Low (25), medium (31), or high (25); tertile of gene expression level. |
Pancreas | |
| Sebastiani (26) |
2006 | 40 | IHC PE |
Low (9) or high (23) on a 0-3 score system; low defined as score <2. |
Pancreas | |
|
| ||||||
| RRM1 | Fujita (24) |
2010 | 40 | qPCR FFPE |
Low (12) or high (28); cutoff determined by recursive descent partition analysis. |
Pancreas |
| Nakahira (33) |
2007 | 18 | QPCR Frozen |
Low (9) or high (9) gene expression level; cutoff at median. |
Pancreas | |
| Giovannetti (25) |
2006 | 81 | qPCR Frozen |
Low (29), medium (25), or high (27) gene expression level; tertile cutoff. |
Pancreas | |
| Nakamura (34) |
2010 | 10 | qDFIHC PE |
Low (6) or high (4) quantitative fluroscence level; cutoff at mean. |
Bile Duct | |
|
| ||||||
| RRM2 | Fujita (24) |
2010 | 40 | qPCR FFPE |
Low (13) or high (27); cutoff determined by recursive descent partition analysis. |
Pancreas |
| Itoi (26) |
2007 | 31 | qPCR EUS- FNAB |
Low (18) or high (13) gene expression level; cutoff at median. |
Pancreas | |
| Giovannetti (25) |
2006 | 81 | qPCR Frozen |
Low (18), medium (33), or high (30) gene expression level; tertile cutoff. |
Pancreas | |
|
| ||||||
| DPD | Kondo (28) |
2011 | 86 | IHC FFPE |
Low (51) or high (35) on a 0-3 score system; high defined as score >=2 in > 30% cells. |
Pancreas |
| Murata (32) |
2011 | 55 | IHC FFPE |
Low (15) or high (40); low defined as positive staining in < 30% cells. |
Pancreas | |
| Komori (27) |
2010 | 13 | IHC FFPE |
Low (6) or high (7) expression index measuring % cell staining; cutoff at median. |
Pancreas | |
IHC = immunohistochemistry; qPCR = real time polymerase chain reaction; FFPE = formalin fixed paraffin embedded tissue; PE = paraffin embedded tissue