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. Author manuscript; available in PMC: 2013 Oct 30.
Published in final edited form as: J Immunol. 2010 Mar 31;184(9):10.4049/jimmunol.0904076. doi: 10.4049/jimmunol.0904076

FIGURE 9.

FIGURE 9

Experimental protocol to test antigen-specific memory T cell responses. In phase I of the experiment two groups of RAG−/− mice were adoptively transferred naïve T cells: RAG−/− animals with or without pre-transfer of Thy1.2 Tregs. After 30 days of reconstitution these mice were infected with 107 attenuated L. monocytogenes bacteria. 10 days after infection the phase I animals were sacrificed and their Thy1.1 T cells were re-transferred into wild-type (Thy1.2/CD45.1 RAG+/+) mice, 3 × 106 per recipient. The new recipients were then either infected with 107 attenuated L. monocytogenes bacteria or not. Magnetic bead enrichment and flow cytometric analysis was used to enumerate Thy1.1 (donor) and Thy1.2 (recipient) IAb-2W1S-specific T cells.