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SDS-PAGE pattern of L .acidophilus ATCC 4356 S-layer protein extracted via different methods. M: molecular weight marker, Lane1, 2: S-layer protein extracted with GHCl (4M) in water and GHCl (4M) in 50 mM Tris-HCl buffer, lane 3: S-protein extracted with Urea (8M), lane 4, 5: S-protein extracted with LiCl (1, 5 M) , lane 6: Lactobacillus casei without S-layer protein as a negative control
