Table 1.

Extraction of Lactobacillus acidophilus ATCC4356 S-layer protein from whole cell bacteria in different incubation conditions.

Incubation condition of L. acidophilus	S-layer protein extraction agent	S-layer protein extracted (mg/mL) (mean ± SEM*, n = 3)	% CV
MRS broth (pH = 6.5) , 37°C in anaerobic jar	GHCl (4M)	0.773 ± 0.012	2.75
MRS broth (pH = 5) , 37°C in anaerobic jar	GHCl (4M)	0.816 ± 0.011	2.47
MRS broth (pH = 6.5) , 37°C in Co2 incubator	GHCl (4M)	0.512 ± 0.006	2.19
MRS broth (pH = 5) , 37°C in Co2 incubator	GHCl (4M)	0.607 ± 0.004	1.23
MRS broth (pH = 6.5) , 45°C in anaerobic jar	GHCl (4M)	0.984 ± 0.004	0.76
MRS broth (pH = 5) , 45°C in anaerobic jar	GHCl (4M)	1.15 ± 0.032	4.93
MRS broth (pH = 6.5) , 45°C in CO2 incubator	GHCl (4M)	0.835 ± 0.008	1.44
MRS broth (pH = 5) , 45°C in CO2 incubator	GHCl (4M)	1.007 ± 0.001	2.49
MRS broth (pH = 6.5) , 37°C in anaerobic jar	Urea (8M)	0.227 ± 0.006	5.13
MRS broth (pH = 5) , 45°C in anaerobic jar	Urea (8M)	0.354 ± 0.007	3.57
MRS broth (pH = 6.5) , 37°C in anaerobic jar	LiCl (1M)	0.088 ± 0.004	8.64
MRS broth (pH = 5) , 45°C in anaerobic jar	LiCl (1M)	0.111± 0.002	3.39
MRS broth (pH = 6.5) , 37°C in anaerobic jar	LiCl (5M)	0.135 ±0.008	11.08
MRS broth (pH = 5) , 45°C in anaerobic jar	LiCl (5M)	0.182 ± 0.004	4.11

SEM* represents s standard error of the mean values of results from three independent experiments