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. 2013 Nov;142(5):523–542. doi: 10.1085/jgp.201311064

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© 2013 Garg et al.

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Figure 4. — Intracellular NaCl loading of oocytes activates a robust I_Slo2.1 in oocytes expressing WT Slo2.1, but not in oocytes expressing S6 mutant channels that did not respond to NFA treatment. (A) Time course of I_Slo2.1 activation induced by leakage of NaCl from recording pipettes in a single oocyte injected with 10 ng WT Slo2.1 cRNA. Currents were elicited with 300-ms pulses applied to 0 mV every 10 s for a total of 5 min, 40 s. The first current (smallest amplitude) was recorded immediately after impalement of the oocyte. The arrow on left indicates 0 current level. (B) Mean time course of I_Slo2.1 activation induced by intracellular NaCl loading for cells recorded 3 d after injection with 10 ng WT Slo2.1 cRNA (n = 12) compared with uninjected cells (n = 12) from the same batch of oocytes. Currents were measured at the end of 300-ms pulses applied to 0 mV every 10 s. (C) NaCl loading activates small currents in oocytes expressing K256A, I263A, and Q273A mutant channels. The right panel shows data with an expanded y axis. (D) P271A and E275A mutant channels are not activated by NaCl loading. The right panel shows data with an expanded y axis. Error bars indicate mean ± SEM.