Fig. 3.

Clonal expansion and intraclonal diversity of a B cell clone isolated from a meningioma. (A) Alignment of CDR3 protein sequences, as well as V-D-J gene segment use, of clonally related TIL-Bs. Amino acid differences are italicized and in bold compared with the CDR3 region encoded by the germline allele. (B) Variable gene segments were aligned at the nucleotide level for 2 clonally related TIL-Bs. Solid vertical lines represent coding mutations that resulted in amino acid replacement, and dashed lines represent silent mutations, compared with the most homologous germline segment.