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. 2013 Aug 26;15(11):1479–1490. doi: 10.1093/neuonc/not110

Fig. 5.

Fig. 5.

Flow cytometric analysis of TIL-Ts and matched PBMCs. TIL-Ts (025, 027, 029) and matched PBMCs were first gated by CD4+ and CD8+ T cell subsets. Then the surface markers CD45RA, CD45RO, PD-1, Tim-3, CD25, and the intracellular marker Foxp3 were used to demarcate particular T cell subsets. (A) Frequency of CD4+ and CD8+ T cell subsets. (B) Frequency of naïve (CD45RA+CD45RO-), antigen (Ag)-experienced memory/effector (CD45RA−CD45RO+) and Treg (CD25+Foxp3+) populations within CD4+ T cell subsets. (C) Frequency of naïve (CD45RA+CD45RO−), antigen-experienced memory/effector (CD45RA−CD45RO+) populations within CD8+ T cell subsets. (D) Frequency of T cells displaying an exhausted phenotype (PD-1+ and Tim-3+) within the CD4+ and CD8+ T cell subsets. Error bars represent SEMs. Statistical differences are shown when significant.