Table 3. Binding kinetics of Cry1Ac WT and mutant toxins to HaALP receptor.
| Toxins | Ka (1/Ms) | Kd (1/s) | KD (M) | Chi2 |
|---|---|---|---|---|
| Cry1Ac WT | 1.680E+5 | 12.91E-4 | 7.681E-9 | 0.52 |
| Q509A | 19.31E+4 | 0.005710 | 2.956E-8 | 11.4 |
| N510A | 2.32E+4 | 0.001355 | 5.838E-8 | 1.65 |
| R511A | 5.58E+4 | 0.001788 | 3.200E-8 | 11.6 |
| Y513A | 6.61E+3 | 0.001469 | 2.229E-7 | 3.60 |
| Triple mutant | 10.25E+3 | 0.002687 | 2.624E-7 | 12.7 |
| Tetra mutant | 0.75E+2 | 1.837E-4 | 2.424E-6 | 16 |
| W545A | 9.63E+2 | 0.006882 | 7.146E-6 | 0.353 |
Ka = Association rate constant
Kd = Dissociation rate constant
KD = Apparent affinity (Kd/Ka)