Table 1. Primer sequences for real-time quantitative polymerase chain reaction analysis of the expression of Runx2, collagen I, alkaline phosphatase (ALP) and osteocalcin (OCN) genes.

Gene	Accession No.	Primers (F = forward; R = reverse)
Akp2 (ALP)	NM_007431	F: 5′- TGCCTACTTGTGTGGCGTGAA -3′;
		R: 5′- TCACCCGAGTGGTAGTCACAATG -3′
Osteocalcin (OCN)	NM_007541	F: 5′- AGCAGCTTGGCCCAGACCTA -3′;
		R: 5′- TAGCGCCGGAGTCTGTTCACTAC -3′
Collagen I	NM_007742	F: 5′- ATGCCGCGACCTCAAGATG -3′;
		R: 5′- TGAGGCACAGACGGCTGAGTA -3′
Runx2	NM_009820	F: 5′- CACTGGCGGTGCAACAAGA -3′;
		R: 5′- TTTCATAACAGCGGAGGCATTTC -3′
β-actin	NM_007393	F: 5′- AGGAGCAATGATCTTGATCTT -3′;
		R: 5′- TGCCAACACAGTGCTGTCT -3′