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A. (upper panels) Analysis of SR-1-RW initiated by addition of ATP only (left, “-GroES”) or ATP and equimolar GroES (right, “+GroES”). Black traces indicate GroEL SR-1 RW only, gray traces indicate SR-1 RW with prebound MDH. B. (lower panels) As in A., but performed using GroEL R231W instead of SR-1 RW in each experiment.
