Table 1. N- and C-terminal sequences of circularly permuted GroEL mutants CP86, CP86-RW, and CP86-C4.

Mutant	C-terminal sequence	N-terminal sequence
CP86	ANDAAGAN 555	1 MAAGDGTT
CP86-RW	ANDAA550	1 MGDGTT
CP86-C4	ANDA CC AN 555	1 MA CCDGTT
Corresponding wild type sequence	81 ANDAAGDGTT90

Underlines and wavy underlines denote the corresponding amino acid sequences in the original wild type sequence. In GroEL CP86-RW, an additional Arg to Trp mutation was introduced at position 149 in the mutant sequence. Amino acids in italics are residues that were introduced as a consequence of the circular permutation protocol, the four cysteine residues introduced to the CP86-C4 sequence are bolded.