Figure 2. AKT inhibitors, MK-2206 decrease cell proliferation of Ctx^R clones. MK-2206 significantly inhibits the proliferation of Ctx^R clones. Ctx^R clones (HC1, HC4, HC8) and Ctx^S parental control (HP) were plated and allowed to adhere for 24 h prior to vehicle (DMSO) or MK-2206 treatment: 5 uM, 7.5 uM or 10 uM. Cell growth was measured at 72 h after drug treatment using the growth proliferation assay described in experimental methods and plotted as a percentage of growth relative to vehicle control cells. Data points are represented as mean ± SEM (n = 4). *p ≤ 0.05, **p ≤ 0.001. Whole cell protein lysates were collected after 24 h treatment and fractionated on SDS-PAGE followed by immunoblotting for the indicated proteins. α-Tubulin was used as a loading control.