Skip to main content
. 2013 Sep 13;6(5):1253–1260. doi: 10.3892/ol.2013.1586

Figure 1.

Figure 1

Expression of B7-H3 in lung cancer cell lines and tumor tissues. (A) PCR analysis of the A549, H460, H1299 and SPCA-1 lung cancer cell lines and tumor tissues using a B7-H3-specific primer. PCR analysis was performed in over six cases of non-small cell lung cancer. The product of ~1500 bp corresponded with the 4IgB7-H3 molecule, whereas the product of 800 bp corresponded with the 2IgB7-H3 gene. (B) Identification of B7-H3 isoforms in the lung cancer cell lines and tumor tissues. The membrane proteins extracted from the A549, H460, H1299 and SPCA-1 cell lines, and tumor tissues were detected by western blotting using a B7-H3 antibody. A band of ~110 kDa represented the 4IgB7-H3 protein, while a protein of ~65 kDa represented the 2IgB7-H3 protein.