Figure 4.

B7-H3 may inhibit the differentiation of monocytes into TAMs in the SPCA-1 cell line. (A) SPCA-1 cell lines highly express B7-H3. (B) Monocytes may express higher levels of MMR and lower levels of HLA-DR when cocultured with the SPCA-1 cell line. (C) Monocytes were cocultured with SPCA-1 cells transfected with pooled B7-H3-specific RNAi. SPCA-1 cells lose the ability to induce the differentiation of monocytes when transfected with B7-H3-specific RNAi. TAMs, tumor-associated macrophages; MMR, macrophage mannose receptor; HLA, human leukocyte antigen.