Figure 4.

Compositions of nonpolymeric and taproot wax fractions of roots from wild-type and far mutant lines. A, Fatty alcohol content, sorted into individual chain lengths, of nonpolymeric fractions from roots of far single, double, and triple mutant lines grown in tissue culture for 4 weeks. Acyl chains were released by acid-catalyzed transmethylation, and hydroxyl groups were silylated before separation by gas chromatography and detection by flame ionization detector. Pentadecanol (C15:0-OH), ω-hydroxy-pentadecanoic acid (ωOH-C15:0), and heptadecanoic acid (C17:0) were used as internal standards for suberin monomer quantification. Mean values are shown in micrograms per milligram delipidated dry residue (DR) ± sd of three replicates. Significance was assessed by Student’s t test (*, P < 0.05; **, P < 0.01; and ***, P < 0.001). B, Total amounts of taproot waxes, AHCs, and free fatty alcohols from roots of 7-week-old far double and far triple mutants grown in a soilless potting medium. C, Total fatty alcohol content (free fatty alcohols plus alcohols from AHCs) from root wax, sorted into individual chain lengths. After taproot waxes extraction, heptadecanoic acid (C17:0), tricosan-1-ol (C23:0-OH), octacosane (C28:0), tridecyl (C13:0) ferulate, and heptadecyl (C17:0) coumarate were added as internal standards, and free hydroxyl groups were silylated before separation by GC-MS. Quantification was based on peaks areas from total ion content chromatograms. Mean values are shown in micrograms per gram fresh weight (FW) of four replicates. Errors bars indicate sd.