Figure 6.

Effect of double and triple far mutations on the permeability of the seed coat. A, Staining pattern in seeds of Col-0 and far mutants incubated in 1% tetrazolium red at 30°C and imaged at the end of 48 h. Bar = 500 µm. B, Time course of formazan produced by reduction of tetrazolium salts by the embryos of the Arabidopsis wild-type (WT) and far double and triple mutants. The higher amount of formazan accumulation indicates increased seed coat permeability (the inset shows the extracted formazans at the 72-h time point). The data are mean value of three replicates shown as measure of A₄₈₅. Error bars indicate sd. C, Seed germination rate of the wild type and far single, double, and triple mutants subjected to varying ABA concentrations. The seed germination percentage was recorded 4 d after placing seeds on MS plates supplemented with ABA at concentrations of 0.5, 1.0, and 10 μm. Values are the means of measurements from two independent experiments using different seed batches and involved three replicates of 100 seeds per plate. The data are shown in percentage of total seeds sown. Errors bars indicate sd.